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Merck

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications (2020-03-01)
Phuong-Uyen C Dinh, Dipti Paudel, Hayden Brochu, Kristen D Popowski, M Cyndell Gracieux, Jhon Cores, Ke Huang, M Taylor Hensley, Erin Harrell, Adam C Vandergriff, Arianna K George, Raina T Barrio, Shiqi Hu, Tyler A Allen, Kevin Blackburn, Thomas G Caranasos, Xinxia Peng, Lauren V Schnabel, Kenneth B Adler, Leonard J Lobo, Michael B Goshe, Ke Cheng
RESUMO

Idiopathic pulmonary fibrosis (IPF) is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation. As fibrosis thickens, the lung tissue loses the ability to facilitate gas exchange and provide cells with needed oxygen. Currently, IPF has few treatment options and no effective therapies, aside from lung transplant. Here we present a series of studies utilizing lung spheroid cell-secretome (LSC-Sec) and exosomes (LSC-Exo) by inhalation to treat different models of lung injury and fibrosis. Analysis reveals that LSC-Sec and LSC-Exo treatments could attenuate and resolve bleomycin- and silica-induced fibrosis by reestablishing normal alveolar structure and decreasing both collagen accumulation and myofibroblast proliferation. Additionally, LSC-Sec and LSC-Exo exhibit superior therapeutic benefits than their counterparts derived from mesenchymal stem cells in some measures. We showed that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.

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Roche
Kit de detecção de morte celular in situ, TMR vermelho, sufficient for ≤50 tests
Sigma-Aldrich
Saponina, for molecular biology, used as non-ionic surfactant
Sigma-Aldrich
Anti-von Willebrand Factor antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Anti-CD81 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution