- Inhibition of glycosphingolipid biosynthesis induces cytokinesis failure.
Inhibition of glycosphingolipid biosynthesis induces cytokinesis failure.
Journal of the American Chemical Society (2011-06-15)
G E Atilla-Gokcumen, A V Bedigian, S Sasse, U S Eggert
PMID21668028
RESUMO
Although cells undergo dramatic shape changes during cytokinesis, the role of the plasma membrane and lipids is poorly understood. We report that inactivation of glucosyl ceramide synthase (GCS), either by RNAi or with the small molecule PPMP, causes failure of cleavage furrow ingression. Using mass-spectrometry-based global lipid profiling, we identify individual lipids that are enhanced or depleted due to GCS inhibition. We show that GCS inhibition results in the mislocalization of actin and the ERM proteins, key cytoskeletal proteins that connect the plasma membrane to the actin cortex. Our data suggest that ceramides participate in mediating the interactions between the membrane and the cortex.
MATERIAIS
Número do produto
Marca
Descrição do produto
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade