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581325-U

Supelco

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Materiais

stainless steel column

Nível de qualidade

100

Agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

linha de produto

Ascentis®

Características

endcapped

fabricante/nome comercial

Ascentis®

embalagem

1 ea of

Extensão da rotulagem

25% Carbon loading

Parâmetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable
LC/MS: suitable

C × D.I.

25 cm × 4.6 mm

área da superfície

450 m2/g

cobertura de superfície

3.7 μmol/m2

Impurezas

<5 ppm metals

matriz

fully porous particle
silica gel high purity, spherical

Grupo ativo da matriz

C18 (octadecyl) phase

tamanho de partícula

5 μm

tamanho de poro

100 Å

operating pH range

2-8

aplicação(ões)

food and beverages

técnica de separação

reversed phase

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Descrição geral

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Características e benefícios

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

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Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
282389-06
282389-05
282389-04
282389-03
282389-02

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Michael Kühl et al.
eLife, 9 (2020-01-22)
Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
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Protocolos

HPLC Analysis of Anthocyanins in Red Wine on Ascentis® C18

-glucoside chloride; Malvidin 3-glucoside; Delphinidin 3-(6-acetylglucoside); Cyanidin 3-(6-acetylglucoside); Petunidin 3-(6-acetylglucoside); Peonidin 3-(6-acetylglucoside); Malvidin 3-(6-acetylglucoside); Malvidin 3-(6-caffeoylglucoside); Petunidin 3-(6-cumarylglucoside); Peonidin 3-(6-cumarylglucoside); Malvidin 3-(6-cumarylglucoside)

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