Y2377

2x YT medium

Powder microbial growth medium

• Sinônimo(s): 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
• Código UNSPSC: 41106225
• NACRES: NA.85

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• esterilidade: non-sterile
• forma: powder
• técnica(s): microbiological culture: suitable
• pH: 6.8-7.2(3.1% solution)
• aplicação(ões): food and beverages; microbiology
• temperatura de armazenamento: room temp
• adequação: nonselective for Escherichia coli; nonselective for coliforms

Descrição

Descrição geral

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Aplicação

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Características e benefícios

2xYT powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Componentes

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Nota de preparo

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Reconstituição

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)