N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Sinônimo(s): p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Fórmula linear: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• Número CAS: 52483-84-8
• Peso molecular: 417.44
• Beilstein: 6553276
• Número CE: 257-949-0
• Número MDL: MFCD00013173
• Código UNSPSC: 12352204
• ID de substância PubChem: 24897623
• NACRES: NA.83

Propriedades

• Nome do produto: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Nível de qualidade: 300
• Ensaio: ≥97% anhydrous basis
• Formulário: powder
• solubilidade: water: 10 mg/mL, clear, colorless to faintly yellow
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• chave InChI: KXNNUYCXODNFBD-UHFFFAOYSA-N

Descrição

Aplicação

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Ações bioquímicas/fisiológicas

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)