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H9789

Sigma-Aldrich

Anti-HMGB2 (HMG2) antibody produced in rabbit

affinity isolated antibody

Sinônimo(s):

Anti-High mobility group box 2, Anti-High mobility group protein 2

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

rat, human, mouse

embalagem

antibody small pack of 25 μL

técnica(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.
indirect immunofluorescence: 1-2 μg/mL using fixed PC12 cultured cells.
western blot: 2-4 μg/mL using PC12 whole cell lysates.

nº de adesão UniProt

P26583

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... HMGB2(3148)
mouse ... Hmgb2(97165)
rat ... Hmgb2(29395)

Descrição geral

The high mobility group (HMG) proteins are subdivided into 3 families based on their functional sequence motif characteristic: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) and the HMGA (formerly HMG-I/Y/C) family. The HMGB protein family, including HMGB1, HMGB2, and HMGB3, are highly conserved (more than 80% amino acid identity), and indistinguishable in their biochemical properties. The high-mobility group box 2 (HMGB2) is a ~25 kDa protein of 209 amino acids that consists of two homologous HMG-boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB2 is restricted mainly to lymphoid organs and testes, although it is widely expressed in embryogenesis.

Imunogênio

synthetic peptide corresponding to amino acid 165-181 of human HMGB2, conjugated to KLH. The corresponding sequence is conserved in mouse and differs by one amino acid in rat.

Aplicação

Anti-HMGB2 (HMG2) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol.

Ações bioquímicas/fisiológicas

The high-mobility group box 1 and 2 (HMGB 1 & 2) proteins participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signalling.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Yasunari Yamanaka et al.
Cell reports, 11(6), 967-976 (2015-05-06)
Long non-coding RNAs (lncRNAs), including natural antisense transcripts (NATs), are expressed more extensively than previously anticipated and have widespread roles in regulating gene expression. Nevertheless, the molecular mechanisms of action of the majority of NATs remain largely unknown. Here, we
Antisense RNA controls LRP1 Sense transcript expression through interaction with a chromatin-associated protein, HMGB2
Yamanaka Y, et al.
Testing, 11(6), 967-976 (2015)
Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2
Ronfani L, et al.
Development, 128(8), 1265-1273 (2001)
The dual role and therapeutic potential of high-mobility group box 1 in cancer
He SJ, et al.
Testing, 8(38), 64534-64534 (2017)
Yu Hu He et al.
Arteriosclerosis, thrombosis, and vascular biology, 37(4), 717-729 (2017-02-12)
In a previous study, we established diabetic and nondiabetic minipig models with coronary artery in-stent restenosis (ISR). Mass spectrometry showed that high-mobility group box (HMGB) 2 level was higher in ISR than in non-ISR tissue from diabetic minipigs. We here

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