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Key Documents

GE28-9505-13

HiScreen SP Fast Flow

Cytiva 28-9505-13

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About This Item

Código UNSPSC:
47101511
NACRES:
NA.56

ligand

sulphopropyl

descrição

Ion Exchanger Type (value)

embalagem

pkg of 1 ea

fabricante/nome comercial

Cytiva 28-9505-13

Parâmetros

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

tamanho do leito

7.7 mm × 100 mm

volume do leito

4.7 mL

D.I. da coluna

7.7 mm

matriz

6% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning

3-14

working range

4-13

adequação

suitable for bioprocess medium

Descrição geral

HiScreen SP FF columns are prepacked with SP Sepharose Fast Flow, a strong cation exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Aplicação

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Características e benefícios

  • SP Sepharose Fast FLow is a strong cation exchanger and IEX Sepharose Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
Store at 4 to 30 °C (20% Ethanol, 0.2 M Sodium Acetate)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

Principles and Standard Conditions for Different Purification Techniques

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ver tudo

Protocolos

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

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