CSE-119

95122019

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: fish unknown/unspecified
• modo de crescimento: Adherent
• cariótipo: Diploid and modal no. 60
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Fish Coho salmon fibroblast

Descrição de linhagem celular

The fish cell line CSE-119 was initiated in 1963 from pooled embryos of Coho salmon (Oncorynchus kisutch). CSE-119, also known as Coho salmon embryo-119, are susceptible to infectious pancreatic necrosis virus (IPNV) but show a lack of sensitivity to infection with viral haemorrhagic septicaemia virus (VHSV) or infectious haematopoietic necrosis virus (IHNV). This might be correlated to the natural resistance to infectious haemotopoietic necrosis in this species. For replication of salmonid viruses CSE-119 cells should be incubated at 18°C.

Aplicação

Isolation of viruses from clinical samples, virus propagation

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days; requires a new flask every passage. Fish cell lines detach easily during transit if the culture is too young. After resuscitation spli

Outras notas

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr J L Fryer, Department of Microbiology, Oregon State University,Corvallis