NP cl.25

Reactivity: Anti-Measles Virus Nucleoprotein, 95051114

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: Sp2/0 Ag-14
• Reatividade: Anti-Measles Virus Nucleoprotein
• técnica(s): cell culture | mammalian: suitable
• Isotipo: IgG2a
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Sp2/0 x Mouse BALB/c spleen

Descrição de linhagem celular

NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.

Imunogênio

Measles virus (strain Hallè)

Aplicação

Diagnosis, study of measles virus infection

Meio de cultura

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

Maintain cultures between 1-5x100,000 cells/ml; 5% CO₂; 37°C

Outras notas

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