IPI-2I

93100622, Porcine intestine, Epithelial-like

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: IPI-2I, 93100622
• fonte biológica: Porcine intestine
• modo de crescimento: Adherent
• cariótipo: Heteroploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Boar Miniature male ileum, SV40 transformed

Descrição de linhagem celular

Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.

Aplicação

Studies of epithelial cell differentiation.

Meio de cultura

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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