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Key Documents

C-12500

Sigma-Aldrich

Human Umbilical Artery Smooth Muscle Cells (HUASMC)

500,000 cryopreserved cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

fonte biológica

human umbilical cord artery

embalagem

pkg of 500,000 cells

morfologia

(smooth muscle)

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origem de linhagem celular

Artery

Aplicação

Primary Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from the arteries of the umbilical cord and are provided in a cryopreserved format. They stain positive for smooth muscle α-actin. Physiological and biochemical processes of vascular diseases can be analyzed with HUASMC in culture.

Qualidade

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

Click here for more information.

Outras notas

Recommended Plating Density: 7500 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Produtos recomendados

Recommended Primary Cell Culture Media:Link

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Artigos

Primary Human Smooth Muscle Cells and Growth Media

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

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