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A7554

Sigma-Aldrich

Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

Sinônimo(s):

Monoclonal Anti-Bovine IgG

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

mouse

conjugado

alkaline phosphatase conjugate

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

secondary antibodies

clone

BG-18, monoclonal

forma

buffered aqueous glycerol solution

reatividade de espécies

bovine (non-reactive to IgM)

não deve reagir com

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

técnica(s)

direct ELISA: 1:25,000
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
western blot (chemiluminescent): 1:100,000

Isotipo

IgG1

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

Especificidade

bovine IgG1 and IgG2

Imunogênio

Purified bovine IgG

Aplicação

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunohistochemistry

Ações bioquímicas/fisiológicas

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

forma física

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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