MABE341-AF488

Anti-Puromycin, clone 17H1, Alexa Fluor^™ 488 Conjugate Antibody

clone 17H1, 0.5 mg/mL, from rat

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• conjugado: ALEXA FLUOR^™ 488
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 17H1, monoclonal
• reatividade de espécies: human
• concentração: 0.5 mg/mL
• técnica(s): immunocytochemistry: suitable
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE341.

Especificidade

Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.

This antibody detects Puromycin-incorporated neosynthesized proteins.

Imunogênio

Puromycin-incorporated neosynthesized proteins at multiple molecular weights

Aplicação

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.

Qualidade

Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor^™ is a registered trademark of Life Technologies.

Descrição-alvo

Refer to Cat. No. MABE341 for observed molecular weight information.

forma física

Protein G Purified

Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable