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MAB10764

Sigma-Aldrich

Anti-SBP-tag Antibody, clone 20 | MAB10764

clone 20, 1.0 mg/mL, from mouse

Sinônimo(s):

streptavidin binding peptide

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

20, monoclonal

reatividade de espécies

human

concentração

1.0 mg/mL

técnica(s)

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Descrição geral

Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.

Especificidade

Demonstrated to react with cells expressing the SBP tag.
This antibody recognizes SBP tag.

Imunogênio

Epitope: Unknown
Linear peptide corresponding to SBP tag.

Aplicação

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Qualidade

Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.

Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.

Descrição-alvo

The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
pTrAP-GFP transfected Hek293 lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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