About This Item
Código UNSPSC:
41106602
NACRES:
NA.54
Produtos recomendados
fabricante/nome comercial
Novagen®
Nível de qualidade
condição de armazenamento
OK to freeze
temperatura de armazenamento
−70°C
Descrição geral
The pCDF-2 Ek/LIC Vector is a LIC-compatible derivative of pCDF-1b. The plasmid contains a strong T7lac promoter and an amino terminal His•Tag coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Componentes
•: 1 µg: Ek/LIC Vector
•: 25 units: T4 DNA Polymerase, LIC-qualified
•: 50 µl: 25 mM EDTA
•: 40 µl: 25 mM dATP
•: 8 µl: Ek/LIC β-gal Control Insert
•: 100 µl: 100 mM DTT
•: 50 µl: 10X T4 DNA Polymerase Buffer
•: 1.5 ml: Nuclease-free Water
•: 5 × 2 ml: SOC Medium
•: 10 µl: Test Plasmid
•: 0.2 ml: BL21(DE3)pLysS Competent Cells
•: 0.2 ml: BL21(DE3) Competent Cells
•: 22 × 50 µl: NovaBlue GigaSingles Competent Cells
•: 25 units: T4 DNA Polymerase, LIC-qualified
•: 50 µl: 25 mM EDTA
•: 40 µl: 25 mM dATP
•: 8 µl: Ek/LIC β-gal Control Insert
•: 100 µl: 100 mM DTT
•: 50 µl: 10X T4 DNA Polymerase Buffer
•: 1.5 ml: Nuclease-free Water
•: 5 × 2 ml: SOC Medium
•: 10 µl: Test Plasmid
•: 0.2 ml: BL21(DE3)pLysS Competent Cells
•: 0.2 ml: BL21(DE3) Competent Cells
•: 22 × 50 µl: NovaBlue GigaSingles Competent Cells
Advertência
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Outras notas
1. Kim, J.S. and Raines, R.T. (1993) Protein Science2, 348–356.
Informações legais
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
10 - Combustible liquids
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica