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Merck
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Key Documents

1.46476

Millipore

ReadyTube® Half Fraser Broth

For enrichment of samples for Listeria sp. and Listeria monocytogenes in accordance to ISO 11190, pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

Sinônimo(s):

Fraser Broth

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About This Item

Código UNSPSC:
41106212

Agency

ISO 11190

Nível de qualidade

forma

liquid (ready-to-use)

Características

ready-to-use

fabricante/nome comercial

ReadyTube®

embalagem

pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

pH

7.2 ( in H2O)

aplicação(ões)

environmental
food and beverages
microbiology

temperatura de armazenamento

2-8°C

adequação

selective for Listeria spp.

Descrição geral

ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Half Fraser broth is a selective enrichment medium designed to help recovery of Listeria sp.

Aplicação

ReadyTube® Fraser broth is used for the primary and secondary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Nota de análise

This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Informações legais

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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