06-519-AF647

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™647 conjugate

from rabbit, ALEXA FLUOR^™ 647

• Sinônimo(s): Cyclic AMP-responsive element-binding protein 1 (pSer133), cAMP-responsive element-binding protein 1 (pSer133), CREB-1 (pSer133), CREB-A (pSer133), htCREB (pSer133), CREB-B (pSer119)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• conjugado: ALEXA FLUOR^™ 647
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: mouse, rat, human
• reatividade da espécie (prevista por homologia): porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology), Xenopus (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)
• técnica(s): immunofluorescence: suitable
• Isotipo: IgG1
• nº de adesão NCBI: NP_604391
• nº de adesão UniProt: P16220
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer133)
• Informações sobre genes: human ... CREB1(1385)

Descrição

Descrição geral

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

Especificidade

Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.

Imunogênio

Epitope: pSer133.

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.

Aplicação

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™647 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.

Qualidade

Evaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.

Descrição-alvo

~43 kDa observed.

forma física

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable