05-1923

Anti-phospho-Threonine Antibody, clone 20H6.1

clone 20H6.1, from mouse

• Sinônimo(s): Threonine
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• tipo de produto de anticorpo: primary antibodies
• clone: 20H6.1, monoclonal
• reatividade de espécies: mouse, human
• reatividade da espécie (prevista por homologia): vertebrates (based on 100% sequence homology), all
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2bκ
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pThr)

Descrição

Descrição geral

Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.

Imunogênio

KLH-conjugated linear peptide corresponding to Threonine.

Aplicação

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

Qualidade

Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.

Descrição-alvo

Various observed

forma física

Format: Purified

Protein G

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Calyculin/okadaic acid treated and untreated A431 cell lysates.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable