05-1403

Anti-Giα2 Antibody, clone L5

clone L5, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: L5, monoclonal
• reatividade de espécies: guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2b
• nº de adesão NCBI: NM_002070.2
• nº de adesão UniProt: P04899
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... GNAI2(2771)

Descrição

Especificidade

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.

This antibody recognizes Giα-2.

Imunogênio

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

Aplicação

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

Qualidade

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

Descrição-alvo

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

Ligação

Replaces: MAB3077

forma física

Format: Purified

Purified

Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

Armazenamento e estabilidade

Maintain at 2-8°C for 1 year from date of receipt.

Nota de análise

Control
Huvec cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable