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Documentos Principais

04-773

Sigma-Aldrich

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

culture supernatant, clone SA19, from rabbit

Sinônimo(s):

Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

100

forma do anticorpo

culture supernatant

tipo de produto de anticorpo

primary antibodies

clone

SA19, monoclonal

reatividade de espécies

rat, chicken, mouse, human

técnica(s)

western blot: suitable

Isotipo

IgG

nº de adesão NCBI

NP_002471.1

nº de adesão UniProt

O14974

Condições de expedição

dry ice

modificação pós-traducional do alvo

phosphorylation (pThr850)

Informações sobre genes

human ... PPP1R12A(4659)

Descrição geral

Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.

Especificidade

Predicted to cross-react with mouse and rat.
Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.

Imunogênio

Epitope: pThr850(Ch) or pThr853(H)
KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Aplicação

Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.

Qualidade

Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).

Descrição-alvo

Doublet at ~130 kDa

Ligação

Replaces: 05-773

Nota de análise

Control
HEK293 cell lysate phosphorylated by ROKα/ROCKII.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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