03-34-0051

Calpeptin

A cell-permeable calpain inhibitor (ID₅₀ = 52 nM for calpain-1; ID₅₀ = 34 nM for calpain-2; ID₅₀ = 138 nM for papain).

• Sinônimo(s): Calpeptin, Benzyloxycarbonylleucyl-norleucinal
• Fórmula empírica (Notação de Hill): C₂₀H₃₀N₂O₄
• Número CAS: 117591-20-5
• Peso molecular: 362.46
• Número MDL: MFCD00155623
• Código UNSPSC: 12352202

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥94% (HPLC)
• forma: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white to off-white
• solubilidade: DMF: 5 mg/mL; DMSO: 5 mg/mL
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C
• InChI: 1S/C20H30N2O4/c1-4-5-11-17(13-23)21-19(24)18(12-15(2)3)22-20(25)26-14-16-9-7-6-8-10-16/h6-10,13,15,17-18H,4-5,11-12,14H2,1-3H3,(H,21,24)(H,22,25)/t17-,18-/m0/s1
• chave InChI: PGGUOGKHUUUWAF-ROUUACIJSA-N

Descrição

Descrição geral

A cell-permeable calpain inhibitor (ID₅₀ = 52 nM for calpain-1; ID₅₀ = 34 nM for calpain-2; ID₅₀ = 138 nM for papain). Inhibits the Ca²⁺-stimulated cleavage of p35 to p25 by calpain. Modulates the processing of β-amyloid precursor protein to β-amyloid (Aβ). Promotes neurite elongation in differentiating PC12 cells. Prevents Ca²⁺-ionophore-induced degradation of actin binding protein and P₂₃₅ in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin, or thrombin. Inhibits the growth of estrogen receptor positive breast cancer cells.

A cell-permeable calpain inhibitor. Inactivates calpain I (ID₅₀ = 52 nM), calpain II (ID₅₀ = 34 nM), and papain (ID₅₀ = 138 nM). Promotes neurite elongation in differentiating PC12 cells. Prevents Ca²⁺-ionophore induced degradation of actin binding protein and P₂₃₅ in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin, or thrombin. Inhibits the growth of estrogen receptor positive breast cancer cells.

Ações bioquímicas/fisiológicas

Cell permeable: yes

ID50 = 52 nM for calpain-1; 34 nM for calpain-2; 138 nM for papain

Product does not compete with ATP.

Reversible: no

Embalagem

Packaged under inert gas

Advertência

Toxicity: Standard Handling (A)

Sequência

Z-Leu-Nle-CHO

Nota de preparo

Dissolve in a minimal amount of DMF or DMSO then add H₂O, buffer or medium just prior to use.

Outras notas

Lee, M.S., et al. 2000. Nature405, 360.
Yamazaki, T., et al. 1997. Biochemistry27, 8377.
Klafki, H., et al. 1996. J. Biol. Chem.271, 28655.
Shiba, E., et al. 1996. Anticancer Res. 16, 773.
Pinter, M., et al. 1994. Neurosci. Lett. 170, 91.
Saito, K., and Nixon, R.A. 1993. Neurochem. Res. 18, 231.
Tsujinaka, T., et al. 1988. Biochem. Biophys. Res. Commun.153, 1201.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable