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Stem Cell Markers and Antibodies

Embryonic Stem Cells

Embryonic stem cells are found early in the development in the inner mass of blastocyst and possess properties of both self-renewal and potency. The embryonic stem cells are pluripotent cells, meaning they can give rise to several different cell types. Pluripotent embryonic stem cells are identified by expression of several pluripotency markers, including OCT4, SOX2 and NANOG. These transcription factors suppress the genes that lead to differentiation and thus maintain the pluripotency of the cells.

nanog-amab-human

Figure 1A.

nanog-amab

Figure 1B.

nanog-amab-ntera

Figure 1C.

Figure 1 (A,B,C). NANOG protein normally is expressed during early embryonal development, and is absent in normal adult tissues. Overexpression of NANOG is observed in testicular embryonal carcinoma (A), shown by IHC staining (B) using monoclonal Anti-NANOG (AMAb91393). Anti-NANOG antibody AMAb91393 also shows nuclear positivity in NTERA-2 cells and band of expected size in the WB assay (C). NTERA-2 cells are pluripotent human embryonal carcinoma cell line, exhibiting biochemical and developmental properties similar to the cells of the early embryo.

During embryogenesis, the embryonic stem cells differentiate and give rise to the three germ layers, including ectoderm, endoderm and mesoderm, from which all the tissues and organs are ultimately formed. For instance, the dorsal part of ectoderm will specialize into neuroectoderm, which later will undergo neurulation, followed by encephalization and will finally develop into the central nervous system. The neural stem cells are multipotent cells that generate various types of neural cells in the process of neurogenesis, including neurons, astrocytes and oligodendrocytes.

sox2-embryo

Figure 2A.

sox2-mouse

Figure 2B.

Figure 2 (A,B). SOX2 is persistently expressed during embryonal development, first in the epiblast of preimplantation embryos, then more predominantly in the central nervous system after gastrulation. Immunohistochemical staining using monoclonal Anti-SOX2 (AMAb91307) shows nuclear positivity in the developing eye (A) and neural tube (B) of mouse embryo.

Adult Stem Cells

Adult (or somatic) stem cells are found in various tissues of the organism after birth. Adult stem cells are lineage-restricted (multipotent) and produce only the cells representing the tissue of origin. Together with progenitor cells, they contribute to normal turnover of cells in adult tissues or may act as a repair system.

IHC Mouse Embryo

Figure 3A.

Adult Rat Retina

Figure 3B.

ICC-IF staining in HEK-293 cells

Figure 3C.

Figure 3 (A,B,C). PAX6 is an essential transcription factor for overall eye development. Immunohistochemical staining using monoclonal Anti-PAX6 (AMAb91372) shows positivity in the developing eye of a mouse embryo (A) and adult rat retina (B). ICC-IF staining in HEK-293 cells shows nuclear immunoreactivity (C).

Transcription Factors Markers
Product No.Product NameDescriptionPricing
HPA055893Anti-MYC antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA055964Anti-KLF2 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AMAB91389Monoclonal Anti-KLF4 antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL5785, purified immunoglobulin, buffered aqueous glycerol solution
HPA040398Anti-KLF5 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AMAB91393Monoclonal Anti-NANOG antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL5810, purified immunoglobulin, buffered aqueous glycerol solution
HPA058267Anti-POU5F1B antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody
HPA015791Anti-SALL4 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA067196Anti-SOX15 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AMAB91307Monoclonal Anti-SOX2 antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL4716, purified immunoglobulin
AMAB91312Monoclonal Anti-SOX3 antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL4725, purified immunoglobulin
HPA062476Anti-TCF3 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA016604Anti-TCL1A antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Ectoderm and Endoderm Markers
Product No.Product NameDescriptionPricing
HPA035827Anti-CHRD antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA067284Anti-FOXJ3 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody
HPA067809Anti-GBX2 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody
AMAB90556Monoclonal Anti-NES antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL0197, purified immunoglobulin, buffered aqueous glycerol solution
HPA061318Anti-NOG antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA000633Anti-OTX2 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AMAB91224Monoclonal Anti-TP63 antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL3748, purified immunoglobulin, buffered aqueous glycerol solution
HPA070751Anti-PAX2 antibody produced in rabbitPrestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody
AMAB91372Monoclonal Anti-PAX6 antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL5414, purified immunoglobulin, buffered aqueous glycerol solution
AMAB91395Monoclonal Anti-TUBB3 antibody produced in mousePrestige Antibodies® Powered by Atlas Antibodies, clone CL5814, purified immunoglobulin, buffered aqueous glycerol solution

For Research Use Only. Not for use in diagnostic procedures.

Unless otherwise stated in the Product(s) specifications, any Antibody product is sold for internal research use only and may not be used for any other purpose, which includes but is not limited to, any commercial, diagnostic, or therapeutic use. Our validation processes pertain only to research uses and do not confirm or assure that our antibodies can be used for any unauthorized uses as set forth herein.

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