Skip to Content
Merck
All Photos(1)

Key Documents

MAK490

Sigma-Aldrich

L-Amino Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

application(s)

food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

genitourinary disorders; cancer

shipped in

wet ice

storage temp.

−20°C

General description

L-amino acids are essential molecules defined by the nature of their side-chain. There are 20 amino acids that are commonly used by most organisms for the production of proteins and other biologically relevant molecules. While amino acids can occur in either the D- or L-enantimer, only the L-form is used by cells.

Features and Benefits

  • Linear detection range: 3.3 to 500 µM (colorimetric assay) and 0.13 to 50 µM (fluorometric assay) for 60 minute reaction.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for L-Amino Acid determination in serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc.

Principle

The L-Amino Acid Assay Kit uses an enzyme-catalyzed oxidation of L-amino acids to convert a dye into a colored or fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 is directly proportional to the L-amino acid concentration in the sample.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service