HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
CONDITIONS
column
Ascentis Express OH-5, 15 cm x 4.6 mm, I.D., 2.7 μm particles (53778-U)
mobile phase
[A] 0.005 M ammonium formate, pH 3.15 with concentrated TFA [B] acetonitrile (30:70, A:B)
flow rate
0.5 mL/min
pressure
841 psi (58 bar)
column temp.
35 °C
detector
ELSD (detector other: Nebulizer 60º C, Evaporator 80º C, Gas flow 1.60 SLM (Standard Liters/ Minute), LED 100%, Smoothing 5.0 S, PMT ( Photomultiplier) Gain 1.0)
injection
10 μL
sample
0.5 mg/mL each prepared in 50:50 Water:Acetonitrile
Description
Analysis Note
This application shows the analysis of sugars by HILIC Chromatography with ELSD detection. The purpose of the work shown here was to develop a HILIC method that could quickly separate mono- and di-saccharides with good sensitivity and reproducibility utilizing the Ascentis Express OH-5 column and an Evaporative Light Scattering Detecctor (ELSD) detector.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany