HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase
acetonitrile
flow rate
1.5 mL/min
pressure
1682 psi (116 bar)
column temp.
30 °C
detector
UV, 280 nm
injection
5 μL
sample
0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol
Description
Analysis Note
Analysis was conducted using standard solution, detection via UV.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany