GC Analysis of Pesticides in Spinach on Equity-1 after SPE using Supelclean™ ENVI-Carb-II/PSA
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
10 g of fresh spinach spiked at 0.2 ppm each pesticide
SPE tube/cartridge
Supelclean ENVI-Carb-II/PSA, 500mg/300mg/6mL (55119-U)
condition
5 mL acetonitrile:toluene (3:1, v/v)
sample addition
1 mL concentrated sample extract
elution
20 mL acetonitrile:toluene (3:1, v/v)
eluate post-treatment
Evaporate eluate to dryness with nitrogen at 40 °C. Reconstitute in 1 mL acetone:hexane (1:1, v/v).
column
Equity-1, 30 m x 0.25 mm I.D., 0.25 μm (28046-U)
oven
50 °C (5 min), 25 °C/min to 125 °C, 10 °C/min to 30 °C (8 min)
inj. temp.
200 °C, aux: 325 °C (8 min)
detector
MSD, scan range 45-450 amu
carrier gas
helium, 0.9 mL/min., constant flow mode
injection
1 μL, splitless (splitter open at 1 min)
liner
4 mm I.D., single taper
Description
Application
Sample Pre-treatment:
Homogenize 10 g of spinach in 10 mL of acetonitrile.
Legal Information
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