Skip to Content

Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Biomaterials science (2015-09-08)

M Hasanzadeh Kafshgari, M Alnakhli, B Delalat, S Apostolou, F J Harding, E Mäkilä, J J Salonen, B J Kuss, N H Voelcker

ABSTRACT

In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Osmium tetroxide, Sealed ampule.

Sigma-Aldrich

Propidium iodide, ≥94.0% (HPLC)

Sigma-Aldrich

D.E.R.^™ 332, used as embedding medium

Sigma-Aldrich

2,4,6-Tris(dimethylaminomethyl)phenol, 95%

Sigma-Aldrich

MISSION^® esiRNA, targeting mouse Abcc1

Sigma-Aldrich

Os EnCat^® 40, extent of labeling: 0.3 mmol/g Os loading

Sigma-Aldrich

Osmium tetroxide, ACS reagent, ≥98.0%

Sigma-Aldrich

Osmium tetroxide, ReagentPlus^®, 99.8%

Sigma-Aldrich

L-Glutamine

Sigma-Aldrich

Dodecenylsuccinic anhydride, mixture of isomers, technical grade, 90%

Sigma-Aldrich

MISSION^® esiRNA, targeting human ABCC1

Sigma-Aldrich

Osmium tetroxide solution, 2.5 wt. % in tert-butanol

Sigma-Aldrich

Osmium tetroxide solution, 4 wt. % in H₂O

Sigma-Aldrich

Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF

Sigma-Aldrich

Epoxy embedding medium, accelerator, ≥95% (NT)

Sigma-Aldrich

Propidium iodide solution

SAFC

L-Glutamine

Sigma-Aldrich

L-Glutamine, BioUltra, ≥99.5% (NT)

Sigma-Aldrich

Osmium tetroxide solution, suitable for electron microscopy, 2% in H₂O

Sigma-Aldrich

Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)

Sigma-Aldrich

Osmium tetroxide solution, suitable for electron microscopy, 4% in H₂O

Sigma-Aldrich

Ethylenediaminetetraacetic acid, 99.995% trace metals basis

Sigma-Aldrich

Triton^™ X-100, laboratory grade

Sigma-Aldrich

L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

Sucrose, Grade I, ≥99% (GC), suitable for plant cell culture

Sigma-Aldrich

Sucrose, ≥99.5% (GC), BioXtra

Sigma-Aldrich

Sucrose, ≥99.5% (GC)

Sigma-Aldrich

Sucrose, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

L-Glutamine, ReagentPlus^®, ≥99% (HPLC)

Sigma-Aldrich

L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source