Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Journal of nanoscience and nanotechnology (2014-04-18)

Ying Sun, Liubin Qin, Dejun Liu, Changhong Liu, Yongwei Sun, Yourong Duan

ABSTRACT

Colloidal gold is extensively used for molecular sensing because that the surface plasmon resonance (SPR) bands are affected by changes in the dielectric properties in the close vicinity of these structures due to the binding of ligands to the corresponding receptor molecules immobilized onto the nanostructures through chemi- or physisorption. We describe a simple method for the detection of Alpha-Fetoprotein-L3 which is a new generation of tumor marker for hepatocellular carcinoma (HCC) based on the aggregation of Lens culinaris agglutinin (LCA) Immobilized Gold Nanoparticles. The LCA conjugated GNPs obtained were 15-20 nm in diameter. The visible color change of the gold nanoparticles from purple to blue on interaction with 100 ng/mL of AFP-L3 is the principle applied here for the sensing of AFP-L3 level. UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. With this method, the protein AFP-L3 can be rapidly detected as demanded for clinical diagnosis.

MATERIALS

Product Number

Brand

Product Description

326542

Sigma-Aldrich

Gold, beads, 1-6 mm, 99.999% trace metals basis

349305

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.997% trace metals basis

326534

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis

265829

Sigma-Aldrich

Gold, foil, thickness 0.5 mm, 99.99% trace metals basis

326585

Sigma-Aldrich

Gold, powder, <10 μm, ≥99.9% trace metals basis

310980

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.99% trace metals basis

265772

Sigma-Aldrich

Gold, powder, <45 μm, 99.99% trace metals basis

349240

Sigma-Aldrich

Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis

349283

Sigma-Aldrich

Gold, wire, diam. 0.1 mm, 99.99% trace metals basis

GF23782350

Gold, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm², permanent mylar 3.5μm support, 99.99+%

GF50454132

Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF38598980

Gold, microfoil, disks, 10mm, thinness 0.5μm, specific density 966μg/cm², permanent mylar 3.5μm support, 99.99+%

GF05586969

Gold, tube, 100mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%

265837

Sigma-Aldrich

Gold, rod, diam. 3.0 mm, 99.99% trace metals basis

326526

Sigma-Aldrich

Gold, wire, diam. 0.127 mm, 99.99% trace metals basis

GF98812097

Gold, rod, 6mm, diameter 6.0mm, as drawn, 99.95%

GF25294620

Gold, microfoil, disks, 10mm, thinness 0.01μm, specific density 20.7μg/cm², permanent mylar 3.5μm support, 99.99+%

GF88214542

Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%

GF26034320

Gold, tube, 100mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF91804271

Gold, insulated wire, 2m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF04885937

Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%

GF46990974

Gold, microfoil, disks, 10mm, thinness 0.05μm, specific density 101.3μg/cm², permanent mylar 3.5μm support, 99.99+%

GF39029968

Gold, rod, 100mm, diameter 2.0mm, as drawn, 99.95%

GF03557336

Gold, rod, 10mm, diameter 6.0mm, as drawn, 99.95%

GF04839767

Gold, tube, 100mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%

GF79016294

Gold, rod, 10mm, diameter 4.0mm, as drawn, 99.95%

GF59949264

Gold, tube, 200mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%

GF96742479

Gold, rod, 50mm, diameter 3.0mm, as drawn, 99.95%

GF72963221

Gold, insulated wire, 0.5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF92307922

Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%