Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

267058

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

221678

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2800, slurry, in H₂O, active catalyst

268283

Sigma-Aldrich

Nickel, powder, <1 μm, 99.8% trace metals basis

267007

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

203904

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

510033

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2400, slurry, in H₂O, active catalyst

GF40564144

Nickel, pellets, 20g, max. size 15mm, 99.99+%

GF97780315

Nickel, foil, 200x200mm, thickness 0.1mm, annealed, 99%

GF38418105

Nickel, foil, 4mm disks, thickness 0.05mm, as rolled, 99.98%

GF44056425

Nickel, microfoil, disks, 10mm, thinness 0.1μm, specific density 93.4μg/cm², permanent mylar 3.5μm support, 99.99+%

GF57196569

Nickel, wire reel, 100m, diameter 0.25mm, annealed, 99%

GF58401660

Nickel, wire reel, 0.1m, diameter 1.0mm, as drawn, 99.99+%

GF01408711

Nickel, foil, 2m coil, thickness 0.15mm, coil width 12mm, annealed, 99%

GF09638321

Nickel, foil, 150x150mm, thickness 0.25mm, as rolled, 99.999%

GF63602467

Nickel, microfoil, disks, 25mm, thinness 0.25μm, specific density 222.55μg/cm², permanent mylar 3.5μm support, 99.99+%

GF88419695

Nickel, insulated wire, 50m, conductor diameter 0.025mm, insulation thickness 0.0035mm, polyimide insulation

GF45623112

Nickel, foil, 50x50mm, thickness 0.5mm, as rolled, 99.999%

GF79244769

Nickel, foil, 300x300mm, thickness 1.6mm, 99%

GF67945887

Nickel, foil, 0.5m coil, thickness 0.25mm, annealed, 99.98%

GF14407927

Nickel, pellets, 100g, max. size 15mm, 99.99+%

GF30576034

Nickel, foil, 150x150mm, thickness 0.5mm, annealed, 99.98%

GF83472329

Nickel, wire reel, 10m, diameter 0.05mm, as drawn, 99.99+%

GF20349435

Nickel, foil, not light tested, 50x50mm, thickness 0.005mm, as rolled, 99.9%

GF36794829

Nickel, foil, 50x50mm, thickness 0.25mm, annealed, 99.98%

GF76364983

Nickel, foil, 25x25mm, thickness 0.5mm, as rolled, 99.999%

GF54196746

Nickel, foil, 200x300mm, thickness 0.15mm, annealed, 99%

GF04087076

Nickel, wire reel, 10m, diameter 0.25mm, as drawn, 99%

GF86231729

Nickel, foil, 4mm disks, thickness 0.05mm, as rolled, 99.99+%

GF16048874

Nickel, microfoil, disks, 10mm, thinness 0.25μm, specific density 222.55μg/cm², permanent mylar 3.5μm support, 99.99+%

GF60595061

Nickel, wire reel, 100m, diameter 0.25mm, as drawn, 99%