Duolink® Proximity Ligation Assay
Duolink® Proximity Ligation Assays (PLA) enable the studying of endogenous Protein-Protein Interactions (PPI) and Post-Translational Modifications, providing a fluorescent signal for a single event that can be visualized by either microscopy or flow cytometry. To study Protein-Protein Interactions, Duolink® PLA technology uses a pair of antibodies labeled with oligonucleotides which when present within 40 nm undertake rolling circle amplification to generate a specific fluorescent signal after the addition of labeled probes.
Available in four different colors, for both flow cytometry and microscopy, Duolink® PLA allows for the visualization of multiple Protein-Protein Interactions per cell. Uncover the opportunities in your research with amplified performance and the benefits of Duolink® PLA technology:
- Visualize protein interactions – both stable and transient
- Visualize post-translational modifications
- Detect endogenous proteins – no overexpression or genetic manipulation
- High specificity – use of two antibodies/probes eliminates false positives
- No special equipment needed – standard immunofluorescence and flow cytometry-based methods available
Duolink® PLA Products
- Duolink® PLA Starter Kits contains all the necessary reagents you need to perform a fluorescent experiment and analyze up to 30 samples.
- Duolink® Probemaker Kits enable the conjugation of a PLA oligo (PLUS or MINUS) to any antibody.
- Duolink® PLA Multicolor Kits allow for multiple PPI visualizations by microscopy.
- Duolink® flowPLA Detection Kits enables flow cytometry applications using four colors: green, orange, red, and far red.
- Duolink® PLA Brightfield Detection contains oligonucleotides labeled with horseradish peroxidase (HRP) allowing use with a light microscope.
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