C-14023
Human Aortic Endothelial Cells (HAoEC)
1 million cells per pellet
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About This Item
UNSPSC Code:
41106514
Recommended Products
biological source
human aorta ( thoracic and abdominal)
packaging
pkg of 1,000,000 cells
morphology
(endothelial)
application(s)
cell analysis (DNA, RNA, and protein)
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
PromoCell Cell Pellets are stored in RNAlater® - an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.
Note: RNAlater® denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater® Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4°C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 μl RNAlater®. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
PromoCell Cell Pellets are stored in RNAlater® - an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.
Note: RNAlater® denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater® Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4°C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 μl RNAlater®. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Cell Line Origin
Aorta
Application
Primary Human Aortic Endothelial Cells (HAoEC) are isolated from the human ascending (thoracic) and descending (abdominal) aorta. They are useful for studying vascular diseases such as thrombosis, atherosclerosis, and hypertension as well as for stent-graft compatibility testing.
Quality
Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Physical form
Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.
Subculture Routine
Click here for more information.
Other Notes
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.
Legal Information
RNAlater is a registered trademark of Ambion, Inc.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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