XNAP2E
Extract-N-Amp™ Plant PCR Kit
sufficient for 100 extractions, sufficient for 500 amplifications
Synonym(s):
Plant direct PCR kit
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About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
usage
sufficient for 100 extractions
sufficient for 500 amplifications
sufficient for 500 reactions
feature
dNTPs included
hotstart
technique(s)
PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
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General description
The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
The PCR reaction mix facilitates amplification directly from the extract by using an antibody-based hot-start DNA polymerase for specific amplification.
Application
Extract-N-Amp™ Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.
It has been used forDNA extraction from fresh mushrooms.
It has been used forDNA extraction from fresh mushrooms.
Features and Benefits
- Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
- No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
- A specially formulated PCR ReadyMix is provided for use with the extracted DNA
- The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
- Suitable for high-throughput plant genetic analysis.
- Lengthy enzymatic digestions are not required, saving time and effort.
- The extracted DNA remains stable at 4 °C for atleast 6 months.
Components
Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
Extraction Solution- E7526-12 mL
Dilution Solution- D5688-12mL
Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml
Collection tubes
Dilution Solution- D5688-12mL
Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml
Collection tubes
Other Notes
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
The Extract-N-Amp™ Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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