HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase
CONDITIONS
column
Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase
methanol
flow rate
1.0 mL/min
pressure
1653 psi (114 bar)
column temp.
30 °C
detector
UV, 280 nm
injection
5 μL
sample
0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol
Description
Remarque sur l'analyse
Analysis was conducted using standard solution, detection via UV.
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany