GC Analysis of Resveratrol in Red Wine on SLB®-5ms after SPME using 85 μm Polyacrylate Fiber
CONDITIONS
sample/matrix
3 mL of red wine (California merlot) diluted 3:1 in 12% ethanol:water
SPME fiber
85 μm polyacrylate FS 23 gauge auto (57294-U)
SPME fiber
85 μm polyacrylate FS 24gauge auto (57305)
SPME fiber
85 μm polyacrylate FS 24gauge manual (57304)
extraction
immersion for 15 min at room temperature while stirring at 400 rpm, fiber gently blotted with a Kimwipe to remove excess water, (then derivatization by immersion in a 4 mL vial containing 5 μL of Sylon-BFT (fresh vial for each analysis than was allowed to equilabrate 60-90 minutes prior to use) for 20 min)
desorption process
280 °C for 2 min
column
SLB-5ms; 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven
100 °C (1 min), 10 °C/min to 325 °C (3 min)
MSD interface
325 °C
scan range
m/z 40-450
carrier gas
helium, 1 mL/min, constant
liner
0.75 mm I.D., SPME
Description
Informations légales
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany