GC Analysis of 1-Phenylethylamine Enantiomers (N-Chloroacetyl Derivatives) on Astec® CHIRALDEX™ B-DM
CONDITIONS
column
Astec CHIRALDEX B-DM, 30 m x 0.25 mm I.D., 0.12 μm (77023AST)
oven
150 °C
inj. temp.
250 °C
detector
FID, 250 °C
carrier gas
helium, 30 psi
injection
1 μL, 80:1 split
sample
3 mg/mL in methylene chloride
peak 1: S(-)-1-phenylethylamine (N-chloroacetyl derivative)
peak 2: R(+)-1-phenylethylamine (N-chloroacetyl derivative)
Description
Remarque sur l'analyse
Can reverse elution order by using CHIRALDEX G-PN, or by using the N-TFA derivative on the B-DM column. Shorter retention and higher alpha observed with the N-chloroacetyl derivative compared to the N-acetyl derivative.
Informations légales
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIRALDEX is a trademark of Sigma-Aldrich Co. LLC