58151AST

CHIRALPAK^® AGP (5 μm) HPLC Columns

L × I.D. 15 cm × 4 mm, HPLC Column

• Code UNSPSC : 41115700
• eCl@ss : 32110501
• Nomenclature NACRES : SB.52

Propriétés

• product name: Chiral - AGP HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
• Matériaux: stainless steel column
• Agence: suitable for USP L41
• Gamme de produits: CHIRALPAK^®
• Conditionnement: pkg of 1 ea
• Fabricant/nom de marque: CHIRALPAK^®
• Paramètres: 137 bar pressure (2000 psi); 20-30 °C temperature; 40 °C max. temp.
• Technique(s): HPLC: suitable
• L × D.I.: 15 cm × 4 mm
• Matrice: fully porous particle
• Groupe de la matrice active: glycoprotein, alpha₁- phase
• Taille des particules: 5 μm
• pH de fonctionnement: 2-8
• Technique de séparation: chiral
• Température de stockage: 2-8°C

Description

Description générale

Hermansson described the use of natural proteins immobilized onto a silica support for chiral separations in 1983 (1). Proteins contain a large number of chiral centers of one configuration, and many other sites that contribute to the general retention process. CHIRALPAK AGP has the broadest range of selectivity of all protein phases currently available. CHIRALPAK AGP phase uses α₁-acid glycoprotein (AGP) as the chiral selector immobilized on spherical 5 μm silica particles. Bonded AGP is a very stable protein that tolerates pure organic solvents, elevated temperatures and pH values from 4 to 7. Operated in reversed-phase mode, CHIRALPAK AGP column separates enantiomers of an extremely broad range of drug substances, such as acids, amines and neutral compounds. The enantioselectivity and retention can easily be regulated by mobile phase pH and ionic strength, and the nature and concentration of the organic modifier.

Features:

• Direct reversed phase resolution of chiral molecules
• Stable with a variety of organic modifiers
• Available in analytical and semi-preparative sizes

(1) Hermansson, J. Direct liquid chromatographic resolution of racemic drugs using a1-acid glycoprotein as the chiral stationary phase. J. Chromatogr. A, 1983, 269, 71-80.

Application

• Evaluation of R- (-) and S- (+) Clenbuterol enantiomers during a doping cycle or continuous ingestion of contaminated meat using chiral liquid chromatography by LC-TQ-MS: This study demonstrates the use of a chiral HPLC column for the separation of clenbuterol enantiomers in biological samples, providing insights into the detection of doping substances or exposure to contaminated meat (Dolores et al., 2019).


• Stereochemical characterization of fluorinated 2-(phenanthren-1-yl)propionic acids by enantioselective high performance liquid chromatography analysis and electronic circular dichroism detection: This research highlights the capability of chiral HPLC columns to differentiate between the enantiomers of complex fluorinated compounds, offering a method for detailed analysis crucial in pharmaceutical studies (Bertucci et al., 2012).

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

CHIRALPAK is a registered trademark of Daicel Corp.