ZRB2482
Anti-Cardiac Troponin T Antibody, clone 1G6 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonyme(s) :
Anti-BLAST-2/CD23 Antibody, clone 3G10 ZooMAb® Rabbit Monoclonal, Cardiac muscle troponin T, TnTc, Troponin T, cardiac muscle, cTnT
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Produit recombinant
expressed in HEK 293 cells
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
1G6, recombinant monoclonal
Description
recombinant, expressed in HEK 293 cells
Gamme de produits
ZooMAb® learn more
Forme
lyophilized
Poids mol.
calculated mol wt 35.92 kDa
Produit purifié par
using Protein A
Espèces réactives
rat, human
Réactivité de l'espèce (prédite par homologie)
monkey, canine, mouse
Conditionnement
antibody small pack of 25
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
Validation améliorée
recombinant expression
Learn more about Antibody Enhanced Validation
Technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG
Séquence de l'épitope
Internal
Numéro d'accès Protein ID
Numéro d'accès UniProt
Conditions d'expédition
ambient
Température de stockage
2-8°C
Informations sur le gène
human ... TNNT2(7139)
Description générale
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Spécificité
Clone 1G6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Cardiac Troponin T. It targets an epitope within 19 amino acids from the internal region.
Immunogène
KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of human cardiac muscle Troponin T.
Application
Quality Control Testing
Evaluated by Western Blotting in Human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cardiac Troponin T in Human skeletal muscle tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin T in Human heart tissue sections.
Affinity Binding Assay:: A representative lot of this antibody bound Cardiac Troponin T peptide with a KD of 6.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution of from a representative lot detected Cardiac Troponin T in L6 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cardiac Troponin T in Human skeletal muscle tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin T in Human heart tissue sections.
Affinity Binding Assay:: A representative lot of this antibody bound Cardiac Troponin T peptide with a KD of 6.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution of from a representative lot detected Cardiac Troponin T in L6 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Description de la cible
Troponin T, cardiac muscle (UniProt: P45379; also known as TnTc, Cardiac muscle troponin T, cTnT) is encoded by the TNNT2 gene (Gene ID: 7139) in human. Troponins are structural proteins in skeletal and cardiac muscle thin filaments. The troponin complex consists of three subunits (I, T, and C) that along with calcium ions plays an important role in the regulation of muscle contraction. Each of these troponins have a specific role in the muscle contraction. Troponin T attaches the troponin complex to the actin filament, troponin C acts as the calcium binding site, and troponin I inhibits interaction with myosin heads in the absence of sufficient calcium ions. Elevated levels of troponin T have been linked to a broad spectrum of cardiomyopathies. Its detection is considered as a more specific test for myocardial damage than creatine kinase but may not always indicate ischemic mechanism of injury. Troponin T is reported to split into two sub-fragments, troponin T1 (TnT1) and troponin T2
Forme physique
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Stockage et stabilité
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Informations légales
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique