ZRB1838
Anti-Phospho-EGFR (Tyr1068) Antibody, clone 3F4 ZooMAb® Rabbit Monoclonal
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recombinant, expressed in HEK 293 cells
Synonyme(s) :
EC:2.7.10.1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Produit recombinant
expressed in HEK 293 cells
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
3F4, recombinant monoclonal
Description
3F4 Clone
Gamme de produits
ZooMAb® learn more
Forme
lyophilized
1 of 4
Cet article | SAB1402559 | SAB1401612 | HPA026846 |
---|---|---|---|
antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
product line Prestige Antibodies® Powered by Atlas Antibodies | product line - | product line - | product line Prestige Antibodies® Powered by Atlas Antibodies |
Gene Information human ... PVRL3(25945) | Gene Information human ... PVRL3(25945) | Gene Information human ... PVRL3(25945) | Gene Information human ... PVRL1(5818) |
Description générale
Spécificité
Immunogène
Application
Evaluated by Western Blotting in lysate from A431 cells treated with EGF.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected EGFR phosphorylated on Tyrosine 1068 in lysate from over-night serum starved A431 cells treated with EGF (50 ng/mL; 10 min.).
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Phospho-EGFR (Tyr1068) in lysate from HeLa cells overnight serum starved and treated with EGF ( (50 ng/mL; 10 min.).
Affinity Binding Assay: A representative lot of this antibody bound Phospho-EGF Receptor-(Tyr1068) peptide with a KD of 1.1 x 10-6 in an affinity binding assay.
Flow Cytometry Analysis: 1 µg from a representative lot detected Phospho-EGFR (Tyr1068) in one million A431 cells treated with EGF (100 ng/mL; 15 min.).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-EGFR (Tyr1068) in human cervical cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Phospho-EGFR (Tyr1068) in A431 cells treated with EGF (200 ng/mL; 10 min.).
Peptide Inhibition Assay: Target band detection in a lysate from A431 cells treated with EGF (50 ng/mL; 10 min) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Description de la cible
Forme physique
Reconstitution
Stockage et stabilité
Autres remarques
Informations légales
Clause de non-responsabilité
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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