H6395

Monoclonal Anti-Haptoglobin antibody produced in mouse

clone HG-36, ascites fluid

• Numéro MDL: MFCD00162410
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: HG-36, monoclonal
• Contient: 15 mM sodium azide
• Espèces réactives: human
• Technique(s): indirect ELISA: 1:4,000
• Isotype: IgG1
• Numéro d'accès UniProt: P00738
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... HP(3240)

Description

Description générale

Monoclonal anti-Human Haptoglobin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Haptoglobin (Hp) is a serum α 2-glycoprotein that exists as a tetramer, composed of two smaller identical α chains and two larger identical β chains. The α-chains are linked to each other by a disulfide bond and each β-chain is similarly linked to an α-chain. Plasma haptoglobin is structurally similar to serum immunoglobulins. Haptoglobin is found in normal plasma at a concentration of 0.3-1.9 mg/ml and accounts for 0.4-2.6% of the total plasma proteins. It is also one of the groups of plasma proteins known as acute phase reactants.

Spécificité

The antibody is specific for the 3 major haplotypes of human haptoglobin (Types 1-1, 2-1 and 2-2). It does not cross-react with human IgG, transferrin, C-reactive protein, α₁-acid glycoprotein, albumin or hemoglobin using ELISA or immunoblotting methods. The antibody recognizes human haptoglobin as purified antigen, in human serum, or when complexed with human hemoglobin in a native or denatured, but non-reduced state.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Haptoglobin antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) to determine plasma haptoglobin (Hp) levels and to probe duplicate dot blot strips for dot blot assay. It has been used in affinity purification of human high-density lipoprotein (HDL) to obtain trypanosome lytic factor (TLF) and also to prepare an affinity resin.

Actions biochimiques/physiologiques

The most characteristic property of haptoglobin is its ability to form stable complexes with extra-corpuscular-free native hemoglobin that has been released during hemolysis and is thus thought to prevent iron loss through urinary excretion and to also prevent renal damage from the hemoglobin. In addition, the native form or normal plasma haptoglobin has been shown to exhibit a broad spectrum of immunosuppressive effects in the immune system. Measurement of haptoglobin levels may therefore be used to monitor the progress of inflammatory reactions and assess the efficiency of test drugs.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable