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Key Documents

GE17-5080-01

HiTrap® rprotein A Fast Flow

Cytiva 17-5080-01, pack of 1 × 5 mL

Synonyme(s) :

HiTrap rProtein, Protein A Fast Flow Column, rProtein A Fast Flow

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

recombinant protein A (E. coli)

Conditionnement

pack of 1 × 5 mL

Fabricant/nom de marque

Cytiva 17-5080-01

Conditions de stockage

(20% Ehtanol)
 (20% Ethanol)

Paramètres

42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Matrice

4% cross-linked agarose

Taille des particules

60-165 μm

Diamètre moyen

90 μm (d50v)

cleaning

2-11

working range

3-10

Capacité

~50 mg binding capacity (human lgG/ml medium)

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Description générale

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.
HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caractéristiques et avantages

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Well-established protein A-based medium used in many approved MAb processes.
  • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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