SK-N-BE(2)

95011815, human nerve, Epitheilioid & neuroblastoid

• Code UNSPSC : 41106514

Propriétés

• product name: SK-N-BE(2), 95011815
• Source biologique: human nerve
• Mode de croissance: Adherent
• Caryotype: Modal no. 44
• Morphologie: Epitheilioid & neuroblastoid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian neuroblastoma

Description de la lignée cellulaire

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

Application

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

Milieu de culture

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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