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A5713

Anticorps monoclonal anti-polyHistidine

Name: Anticorps monoclonal anti-polyHistidine
Brand: SIGMA

clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins

Synonyme(s) :

Anticorps monoclonal anti-His-tag, Anticorps monoclonal anti-épitope marqueur 6 His, Anticorps monoclonal anti-épitope marqueur HHHHHH, Anticorps monoclonal anti-étiquette histidine, Anticorps monoclonal anti-étiquette 6xHis, Anticorps monoclonal anti-étiquette Hexa His, Anticorps monoclonal anti-étiquette His6, Anticorps monoclonal anti-étiquette Poly-His

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About This Item

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.56

Source biologique

mouse

Conjugué

agarose conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

HIS-1, monoclonal

Forme

PBS suspension

Isotype

IgG2a

Capacité

15-30 nmol/mL binding capacity

Adéquation

suitable for purification of HIS tagged recombinant proteins

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.

Spécificité

Cet anticorps reconnaît la polyhistidine de synthèse ainsi que les formes réduites natives ou dénaturées des protéines marquées à l'hexahistidine exprimées dans certains vecteurs.

Immunogène

Protéine de fusion recombinante marquée à la polyhistidine.

Application

Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Forme physique

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Notes préparatoires

Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.

Informations légales

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Directed evolution of proteins in vitro using compartmentalization in emulsions.

Eric A Davidson et al.

Current protocols in molecular biology, Chapter 24, Unit 24-Unit 24 (2009-07-04)

This unit describes a protocol for the directed evolution of proteins utilizing in vitro compartmentalization. This method uses a large number of independent in vitro transcription and translation (IVTT) reactions in water droplets suspended in an oil emulsion to enable

MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.

Dong Meng et al.

The Journal of biological chemistry, 284(17), 11425-11435 (2009-01-21)

betaArrestin is a multifunctional signal scaffold protein. Using SPOT immobilized peptide arrays, coupled with scanning alanine substitution and mutagenesis, we show that the MAPK kinase, MEK1, interacts directly with betaarrestin1. Asp(26) and Asp(29) in the N-terminal domain of betaarrestin1 are

The phosphorylation of Hsp20 enhances its association with amyloid-β to increase protection against neuronal cell death.

Ryan T Cameron et al.

Molecular and cellular neurosciences, 61, 46-55 (2014-05-27)

Up-regulation of Hsp20 protein levels in response to amyloid fibril formation is considered a key protective response against the onset of Alzheimer's disease (AD). Indeed, the physical interaction between Hsp20 and Aβ is known to prevent Aβ oligomerisation and protects

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