A1559
Monoclonal Anti-Biotin–Agarose antibody produced in mouse
clone BN-34, purified immunoglobulin, PBS suspension
Synonyme(s) :
Monoclonal Anti-Biotin
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
mouse
Produit recombinant
expressed in mouse cell line
Conjugué
agarose conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
BN-34, monoclonal
Forme
PBS suspension
Ampleur du marquage
2 mg antibody per mL resin
Technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1
Capacité
0.15-0.3 μmol/mL, agarose binding capacity (biotin-ligand conjugate)
Application(s)
research pathology
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
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Catégories apparentées
Description générale
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Immunogène
Biotin-KLH conjugate
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Monoclonal Anti-Biotin-Agarose antibody produced in mouse has been used in:
- pulldown assay
- coimmunoprecipitation
- chromatin immunoprecipitation
- purification of biotinylated compounds and biotin-containing compounds
Mouse monoclonal anti-biotin antibody was used to identify by western blot S-nitrosylated proteins that had been labeled with a biotin moeity based on the biotin-switch method. No reactivity was visible on the blots when the biotin switch method was inhibited by treatment with 100 mM dithiothreitol showing that this antibody shows no unspecific cross reactivity. The mouse monoclonal anti-biotin antibody does cross react with Arabidopsis plant by western blot, no cross reactivity with unbiotinylated proteins.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Forme physique
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Notes préparatoires
Prepared using purified antibody coupled to cyanogen bromide activated agarose
Autres remarques
Polyclonal anti-biotin antibodies were shown to react with in vivo biotinylated proteins but not monoclonal anti-biotin antibodies.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Emmanouil Rampakakis et al.
Nucleic acids research, 37(17), 5714-5724 (2009-07-30)
Initiation of DNA replication involves the ordered assembly of the multi-protein pre-replicative complex (pre-RC) during G(1) phase. Previously, DNA topoisomerase II (topo II) was shown to associate with the DNA replication origin located in the lamin B2 gene locus in
A two-step strategy to visually identify molecularly imprinted polymers for tagged proteins
Brandis A, et al.
Journal of Separation Science, 40(16), 3358-3367 (2017)
F J Germino et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(3), 933-937 (1993-02-01)
We describe an in vivo approach for the isolation of proteins interacting with a protein of interest. The protein of interest is "tagged" with a portion of the biotin carboxylase carrier protein (BCCP), encoded on a specially constructed plasmid, so
Transient dsDNA breaks during pre-replication complex assembly
Rampakakis E and Zannis-Hadjopoulos M
Nucleic Acids Research, 37(17), 5714-5724 (2009)
Namrata D Udeshi et al.
Nature methods, 14(12), 1167-1170 (2017-10-19)
Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment
Contenu apparenté
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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