137264

HEPES

EMPROVE^® EVOLVE

• Synonyme(s) : Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
• Formule empirique (notation de Hill): C₈H₁₈N₂O₄S
• Numéro CAS: 7365-45-9
• Poids moléculaire : 238.30
• Numéro Beilstein : 883043
• Numéro MDL: MFCD00006158
• Code UNSPSC : 12352200

Propriétés

• Source biologique: synthetic
• Niveau de qualité: 400
• Gamme de produits: EMPROVE^® EVOLVE
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Impuretés: Endotoxin, microbial, and trace metals; tested
• pH: 4.7-5.3; 6.8-8.2
• Plage de pH utile: 6.8-8.2
• pKa (25 °C): 7.5
• pKa: 7.5
• Adéquation: suitable for manufacturing use
• Activité étrangère: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• Température de stockage: 2-30°C
• Chaîne SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clé InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Informations légales

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable