MHA000P2N

Bouillon nutritif Wallerstein

ready-to-use, ampule of 2 mL, for molds, for bacteria, for yeasts, suitable for microbiology

• Synonyme(s) : Bouillon nutritif WL, Bouillon WLN
• Code UNSPSC : 41106225
• eCl@ss : 32160801
• Nomenclature NACRES : NA.74

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Durée de conservation: 12 mo.
• Caractéristiques: ready-to-use
• Conditionnement: ampule of 2 mL; pack of 50 tests
• Couleur: slightly opalescent green-blue
• Entrée: sample type bottled water; sample type beer; sample type sport drink; sample type cider; sample type food(s); sample type beverage(s); sample type wine; sample type cosmetics; sample type soft drink; sample type beverage(s)
• pH: 5.5±0.2 (25.0 °C)
• Application(s): environmental; food and beverages; industrial qc; microbiology
• Compatibilité: for use with MF-method; for use with Microfil^®; for use with MicropreSure^®; for use with Milliflex^®
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Adéquation: bacteria; molds; yeasts

Description

Description générale

Wallerstein Nutrient Broth is recommended for the isolation and cultivation of yeasts, molds and bacteria that grow in a low pH environment mainly in the beverage industry. Yeast extract in the medium is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is a source of carbohydrates. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride, and ferric chloride are essential ions to help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromocresol green is the pH indicator. The WL Nutrient medium plate incubated under aerobic conditions enables the growth of mainly yeast colonies, acetic acid bacteria, flavobacterium, proteus and thermophilic bacteria and under anaerobic condition enables the growth of lactic acid bacteria and Pediococcus. Bacterial colonies appear as non-pigmented to white, with a smooth or rough texture. Yeast appears as large creamy to white colonies.

Application

Le bouillon nutritif WLN est utilisé pour détecter les bactéries, levures et moisissures dans les moûts, bières et autres produits de fermentation. {12}

Caractéristiques et avantages

• Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
• They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Caractéristiques

Temps d′incubation : 48–72 h

Température d′incubation : 35 °C ± 2 pour les bactéries ; 30 °C ± 2 pour les levures et moisissures

Remarque sur l'analyse

Les colonies bactériennes peuvent être non pigmentées à blanches, avec une texture lisse ou rugueuse. Les levures se présentent sous forme de grandes colonies blanc crème.

Micro-organismes ciblés : Bactéries lactiques, levures et moisissures

Organismes de contrôle qualité
L. fermentum (ATCC 9338)

S. cerevisiae (ATCC 9763)

Informations légales

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable