MABT529

Anti-Integrin beta-1 (CD29) Antibody, clone 13C4.1

clone 13C4.1, from mouse

• Synonyme(s) : Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 13C4.1, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1λ
• Numéro d'accès NCBI: NP_002202
• Numéro d'accès UniProt: P05556
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITGB1(3688)

Description

Description générale

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Spécificité

Clone 13C4.1 targets an epitope within the membrane-proximal extracellular region present in all five spliced isoforms of human Integrin beta-1 reported by UniPort (P05556).

Immunogène

GST-tagged recombinant human Integrin beta-1 fragment corresponding to the membrane-proximal extracellular region.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Integrin beta-1 in human breast cancer, cerebral cortex, and lung tissue sections.

Research Category
Cell Structure

This mouse monoclonal Anti-Integrin beta-1 (CD29), clone 13C4.1, Cat. No. MABT529, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of Integrin beta-1.

Qualité

Evaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Integrin beta-1 in 10 µg of U937 cell lysate.

Description de la cible

~150 kDa observed. Target band size appears larger than the calculated molecular weights of 88.42/87.45/91.62/91.03/88.88 kDa (isoform Beta-1A/Beta-1B/Beta-1C/Beta-1C-2/Beta-1D pro-form) and 86.19/85.22/89.40/88.81/86.66 kDa (mature isoform Beta-1A/Beta-1B/Beta-1C/Beta-1C-2/Beta-1D) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Liaison

Replaces: 04-1109

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable