MABN707
Anti-DCX Antibody, clone 2G5
clone 2G5, from mouse
Synonyme(s) :
Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
2G5, monoclonal
Espèces réactives
mouse, human
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Dcx(13193)
Description générale
Doublecortin (DCX) or alternatively known as Doublin or Lissencephalin-X (Lis-X) and encoded by the human gene named DCX/DBCN/LISX is a microtubule associated protein that is required for the initial steps of neuronal dispersion and lamination during cortex brain development. Expressed by neuronal precursor cells and immature neurons, DCX expression is decreased by the time neurons are mature and express the mature neuronal marker NeuN. Thus DCX expression is a hallmark of developing neurons and neurogenesis. DCX expression is localized in the cytoplasm and along microtubule tracks. DCX appears to stabilize microtubules and causes bundling of them in the cytoplasm. In disease, DCX is the cause for an X-linked lissencephaly and subcortical laminar heterotopia, a disorder that is characterized by a “smooth” brain with few folds like normal brains and poorly formed cortical layers as well as other abnormalities. Besides developmental effects DCX may potentially help in the suppression of gliomas because in brain tumor stem cells (BTSC) self-renewal ability was significantly reduced in cells expressing DCX compared to control cells. EMD-Millipore’s Anti-DCX mouse monoclonal has been tested in western blot against recombinant protein as well as mouse heart lysate. The antibody has also been successfully tested in paraffin embedded immunohistochemistry on normal human brain and kidney cancer tissue sections as well as in fluorescent immunocytochemistry on HepG2 cells in culture and flow cytometry on SK-N-SH cells in culture and direct ELISA on varying concentrations of antigen.
Immunogène
Purified recombinant fragment of human DCX expressed in E. Coli.
Application
Detect Doublecortin using this mouse monoclonal antibody, Anti-DCX Antibody, clone 2G5 validated for use in western blotting, IHC, ICC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in human brain and kidney cancer tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in HepG2 cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DCX in non serum starved SK-N-SH cells.
Optimal working dilutions must be determined by end user.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in HepG2 cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DCX in non serum starved SK-N-SH cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Qualité
Evaluated by Western Blotting in Mouse heart lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DCX in mouse heart lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DCX in mouse heart lysate.
Description de la cible
~50 kDa observed. Uncharacterized bands may appear in some lysate(s).
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
Mouse heart lysate
Mouse heart lysate
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Vincent Quoc-Huy Trinh et al.
The Journal of pathology, 258(1), 69-82 (2022-06-11)
The development of neural structures within tumors is now considered vital for carcinogenesis. However, the time course of this development in human pre-invasive neoplasia has been incompletely described. Therefore, we performed a detailed analysis of nerves across the neoplastic spectrum
Christopher T Rhodes et al.
Cell cycle (Georgetown, Tex.), 17(3), 377-389 (2018-02-13)
Histone methyltransferases (HMTs) are present in heterogeneous cell populations within the adult brain including neurogenic niches. Yet the question remains whether loss of HMTs and the resulting changes in histone methylation alter cell fate in a region-specific manner. We utilized
Wenshu Zhou et al.
Stem cell research & therapy, 12(1), 174-174 (2021-03-14)
Spinal cord injury (SCI) is a debilitating medical condition that can result in the irreversible loss of sensorimotor function. Current therapies fail to provide an effective recovery being crucial to develop more effective approaches. Mesenchymal stem cell (MSC) exosomes have
Michael R Foret et al.
Frontiers in genetics, 5, 252-252 (2014-08-16)
Histone 3 Lysine 9 (H3K9) methylation is known to be associated with pericentric heterochromatin and important in genomic stability. In this study, we show that trimethylation at H3K9 (H3K9me3) is enriched in an adult neural stem cell niche- the subventricular
Richard S Sandstrom et al.
Scientific reports, 4, 5371-5371 (2014-06-21)
Histone 3 lysine 4 trimethylation (H3K4me3) is known to be associated with transcriptionally active or poised genes and required for postnatal neurogenesis within the subventricular zone (SVZ) in the rodent model. Previous comparisons have shown significant correlation between baboon (Papio
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