MABE1823

Anti-AsCpf1 Antibody, clone 3D3-F7

clone 3D3-F7, from mouse

• Synonyme(s) : EC 3.1.27.2, AsCpf1, CRISPR-associated endonuclease Cpf1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3D3-F7, monoclonal
• Espèces réactives: bacteria, Acidaminococcus
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès UniProt: U2UMQ6
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

CRISPR-associated endonuclease Cas12a (UniProt: U2UMQ6; also known as EC: 3.1.27.2, AsCpf1, CRISPR-associated endonuclease Cpf1) I encoded by the Cas12a (also known as cpf1) gene in Acidaminococcus species. CRISPR is an adaptive immune system that provides protection against mobile genetic elements, such as viruses, transposable elements, and conjugative plasmids. CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. They are transcribed and processed into CRISPR RNA (crRNA). Cas12a, an endonuclease from the type V-A CRISPR system, has several unique characteristics when compared to Cas9 system that increase the utility of CRISPR-based gene editing. Cas12a mediated cleavage utilizes a single and short CRISPR RNA) without requiring trans-activating crRNA. Cas12a recognize a 5′ T-rich protospacer adjacent motif (PAM, TTTN for this specific enzyme), unlike Cas9 enzymes which recognize 3′ G-rich PAMs, thus this enzyme increases the possibilities for genome editing. The endonuclease activity of Cas12a results in staggered 4-base 5′ overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively.

Spécificité

Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.

Immunogène

His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.

Application

Anti-AsCpf1, clone 3D3-F7, Cat. No. MABE1823, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cpf1 and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Qualité

Evaluated by Western Blotting in HEK293 cells expressing HA-tagged AsCpf1.

Western Blotting Analysis: 1 µg/mL of this antibody detected AsCpf1 in lysate from HEK293 cells expressing HA-tagged AsCpf1.

Description de la cible

~151 kDa observed; , 151.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.