MABC946

Anti-PKCζ Antibody, clone 14B12.3

clone 4B12.3, from mouse

• Synonyme(s) : Protein kinase C zeta type, nPKC-zeta, PKC-zeta, PKCζ
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 4B12.3, monoclonal
• Espèces réactives: mouse, human, rat
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_002735
• Numéro d'accès UniProt: Q05513
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PRKCZ(5590) ; mouse ... Prkcz(18762) ; rat ... Prkcz(25522)

Description

Description générale

Protein kinase C zeta type (EC 2.7.11.13; UniProt Q05513; also known as nPKC-zeta, PKC-zeta) is encoded by the PRKCZ (also known as PKC2) gene (Gene ID 5590) in human. Protein kinase Cs (PKCs) are threonine/serine kinases that play important roles in cellular signal transductions. PKC isozymes are classified into three major classes based on their domain compositions and activator requirements. The conventional PKCs (cPKCs) include the alpha, beta I, beta II, and gamma isoforms. The novel PKCs (nPKCs) include the delta, epsilon, eta, and theta isoforms. Atypical PKCs (aPKCs) include the lambda (mouse ortholog to human PKC-lambda is called PKC-iota) and zeta isoforms. Both cPKCs and nPKCs bind diacylglycerol (DAG) via their C1 domain, while only cPKCs, but not nPKCs bind Ca+2 via their C2 domain. The aPKCs do not bind either DAG or calcium for activity. All three classes of PKCs contain a pseudosubstrate sequence in their N-terminal region that keeps the substrate-binding cavity occupied via intramolecular interaction when the enzymes are in their inactive conformation. Conformation change upon enzyme activation frees the substrate-binding site.

Spécificité

Expected to react with all three spliced isoforms of human PKC-zeta.

Immunogène

Epitope: C-terminal region

GST-tagged recombinant human PKCζ C-terminal fragment.

Application

Anti-PKCζ Antibody, clone 14B12.3 is an antibody against PKCζ for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PRKCZ in 10 µg of PC12 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PRKCZ in human cerebral cortex, human cerebellum and mouse brain tissues.

Qualité

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PKCζ in 10 µg of mouse brain tissue lysate.

Description de la cible

~70 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable