MAB3806
Anti-ATM phosphoSer1981 Antibody, clone 10H11.E12
clone 10H11.E12, Chemicon®, from mouse
Synonyme(s) :
Ataxia Telangiectasia Mutated
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
10H11.E12, monoclonal
Espèces réactives
mouse, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pSer1981)
Informations sur le gène
human ... ATM(472)
Description générale
ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). Ataxia telangiectasia (AT) is a debilitating neurodegenerative disease that occurs early in childhood, resulting in ataxic movements and speech defects caused by cerebellar degeneration. The underlying cause of the disease is a biochemical dysfunction in the cellular response to specific types of DNA damage, correlated with mutations in the protein kinase ATM (Ataxia telangiectasia mutated). ATM not only is key in the signaling cascade that responds to DNA double-stranded breaks, but it also controls cell-cycle checkpoints and is therefore important in cancer prevention.
Spécificité
Reacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
Immunogène
Epitope: phosphoSer1981
Synthetic peptide corresponding to amino acids 1974-1988 of human ATM (SLAFEEG[pS]QSTTISS).
Application
Anti-ATM Antibody, phosphoSer1981, clone 10H11.E12 is a Mouse Monoclonal Antibody for detection of ATM also known as Ataxia Telangiectasia Mutated & has been validated in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western blot: Detects a 370 kDa protein in crude extracts from irradiated human foreskin or mouse 3T3L1 cells.
Immunocytochemistry: Foci are detected in irradiated human and mouse fibroblasts.
Immunoprecipitation: The antibody immunoprecipitates ATM from irradiated human and mouse cells.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: Foci are detected in irradiated human and mouse fibroblasts.
Immunoprecipitation: The antibody immunoprecipitates ATM from irradiated human and mouse cells.
Optimal working dilutions must be determined by end user.
Forme physique
Format: Purified
Purified immunoglobulin presented as a liquid in 0.02M phosphate buffer containing 0.25M NaCl and 0.1% sodium azide.
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
This antibody and certain aspects of its use are disclosed and claimed in pending U.S. Patent Applications published as U.S. Patent Publication Nos. 2003/0077661 and 2003/0157572.
This antibody and certain aspects of its use are disclosed and claimed in pending U.S. Patent Applications published as U.S. Patent Publication Nos. 2003/0077661 and 2003/0157572.
Remarque sur l'analyse
Control
Irradiated normal Human fibroblasts (no reactivity against non-irradiated cell extracts)
Irradiated normal Human fibroblasts (no reactivity against non-irradiated cell extracts)
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
P Khongkow et al.
Oncogene, 33(32), 4144-4155 (2013-10-22)
FOXM1 is implicated in genotoxic drug resistance but its mechanism of action remains elusive. We show here that FOXM1-depletion can sensitize breast cancer cells and mouse embryonic fibroblasts (MEFs) into entering epirubicin-induced senescence, with the loss of long-term cell proliferation
ATM-mediated phosphorylation activates the tumor-suppressive function of B56?-PP2A.
G P Shouse,Y Nobumori,M J Panowicz,X Liu
Oncogene null
Christopher J Bakkenist et al.
Nature, 421(6922), 499-506 (2003-01-31)
The ATM protein kinase, mutations of which are associated with the human disease ataxia-telangiectasia, mediates responses to ionizing radiation in mammalian cells. Here we show that ATM is held inactive in unirradiated cells as a dimer or higher-order multimer, with
Development of a high-content high-throughput screening assay for the discovery of ATM signaling inhibitors.
Bardelle, C; Boros, J
Journal of Biomolecular Screening null
Sarah Osterwald et al.
Journal of cell science, 128(10), 1887-1900 (2015-04-25)
The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres.
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